STUDY ON APPLYING RT-PCR METHOD IN IDENTITY JAPANESE ENCEPHALITIS LIVE ATTENUATED VACCINE

Abstract

Identity antigen in vaccine is one of the requests for quality control of vaccine originated from virus or virus particles in which Reversed transcription Polymerase Chain Reaction method (or known as RT-PCR) is used widly. Electrophoresis is carried out for PCR product and read the result under UV light. Therefore, we perform a study to apply RT-PCR method in identity test of a live, attenuated Japanese encephalitis vaccine (IMOJEV) from Sanofi Pasteur and evaluate specific indicator to aim to reduce procedure, timing, chemicals and consumables expense. The results were shown that primers were designed to fit and specific to Japanese encephalitis (JE) virus. The size of RT-PCR product was 458 bp, electrophoresis bands were clear, specific to JE virus and unspecific to other live virus that parallelly joined in this study. Therefore, we can conclude that the identity procedure for IMOJEV vaccine was carried out and optimized based on condition and chemicals of Department of Quality control of viral vaccine. Research result shows the suitability and specificity of primers for JE vaccine and they can be applied for future study at Department of Quality control of viral vaccine in National Institute for Control of Vaccines and Biologicals (NICVB).