COMPLETING THE CHROMOSOME TESTING PROCESS FROM FETAL UMBILICAL CORD MESENCHYMAL STEM CELLS

Abstract

 Stem cells from the fetal umbilical cord play a crucial role in treating various diseases, including cardiovascular diseases, neurological disorders, musculoskeletal conditions, and more. However, to ensure treatment effectiveness, stem cells must meet certain standards, including genetic material criteria. Currently, the most fundamental method for testing the integrity of stem cell genetic material at the cellular level is chromosome testing. This research was conducted to complete the chromosome testing process for mesenchymal stem cells from fetal umbilical cord tissue. This cross-sectional study involved 12 umbilical cord tissue samples at the Stem Cell and Genetics Center, Buu Dien Hospital, and Hanoi Medical University. The research results have finalized the procedure from the process of cell culture, harvesting, mitotic arrest, hypotonic shock to the steps of slide staining and chromosome evaluation. The cell division process at metaphase using Colcemid at a final concentration of 200 ng/ml with a 3-hour incubation time, hypotonic shock with a 0.4% KCl solution combined with distilled water and serum in a 4:3:1 ratio, cell fixation with Carnoy's solution, slide staining using the G-banding method, and karyotype analysis of all 12 samples showed normal results of 2n=46 chromosomes. The research results are significant in contributing to the evaluation of stem cell quality for future disease treatments