EXPRESSION ENZYME CONVERT GLYCEROL TO DIHYDROACETONE FROM BACTERIA
DOI:
https://doi.org/10.56086/jcvb.v4i3.163Từ khóa:
Gene expression, protein purification, bacterial enzymeTóm tắt
Glycerol dehydrogenase plays an important role in the glycerol metabolic pathway. During oxidation, glycerol is converted to dihydroxyacetone (DHA), and it is the first step in this cycle. Nowadays, DHA is synthesized because of its value and application in many industries. In this study, we focused on expressing the enzyme capable of converting glycerol into DHA on the bacteria strain Klebsiella pneumoniae. The results of gene expression and purification of recombinant protein encoding the enzyme GlyDH are presented in this study. With an in vitro cell culture volume of 1 liter and high expression and purification capabilities, it shows great potential in practical application.
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Đã Xuất bản
2024-09-05
Cách trích dẫn
Nguyen Quyet Thang, Gyeong Soo Ko, Doan Huu Thien, Duong Thi Cam Le, Dam Thi Lieu, Pham Quang Minh, Canh Huyen Trang, Bui Thanh Tung, Kang Wonchull, & Jang Jin Kuk. (2024). EXPRESSION ENZYME CONVERT GLYCEROL TO DIHYDROACETONE FROM BACTERIA. JOURNAL OF CONTROL VACCINES AND BIOLOGICALS, 4(3). https://doi.org/10.56086/jcvb.v4i3.163
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