VALIDATION OF KLEBSIELLA OXYTOCA TEST IN MICE BY CULTURE METHOD

Abstract

Process validation is one of the important steps to assess the suitability and confirm the validity of the method, to ensure the reliability of the test results. Klebsiella oxytoca is a Gram-negative bacteria that causes opportunistic disease in humans and rodents, often found in the digestive tract and can grow well in culture media. This study was conducted to validate the process of identifying Klebsiella oxytoca in ICR white mice by culturing on DHL, MacConkey, SIM, blood agar, Ure indole medium, Oxydase test and identifying with the bacterial identification machine BD Phoenix. The study used the experimental descriptive method in the laboratory. The process of identifying Klebsiella oxytoca was optimized based on the testing process of the Laboratory Animal Center of Mahidol University, Thailand, which is AAALAC certified. Therefore, the evaluation was conducted in part with two criteria: Specificity and strength