RESEARCH ON THE DISINFECTION EFFICIENCY OF HYDROGEN PEROXIDE, CHLORAMIN B AND BENKOCID AGAINST SOME BACTERIAL AND FUNGAL AGENTS AT THE EXPERIMENTAL ANIMAL DEPARTMENT, NICVB

Abstract

Today, various disinfectants with different mechanisms of action have been researched and developed to prevent the spread of pathogens. The disinfectants that have been used at the Experimental Animal Department, NICVB such as Chloramin B 2%, Benkocid 0.25% and Hydrogen peroxide 5% - the newly used one - need to be evaluated for efficiency against a number of bacteria and fungi. Selected bacterial and fungal agents were neutralized at different times at the Experimental Animal Department’s laboratory from April to November 2023. After a 5-minute exposure, Hydrogen peroxide 5%, Chloramin B 2%, Benkocid 0.25% were shown to be effective in disinfecting bacterial strains Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis at a concentration of 10⁵ CFU/ml; while the exposure period required to disinfect Candida albicans 10⁵ CFU/ml is 15 minutes. To conclude, the optimal exposure period of the disinfectants to the selected microorganism strains in the study was 30 minutes.